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Antimicrobial activity of AWME3 against the A. baumannii ATCC 19606 strain. The IZ values were measured after 12 h and 24 h at 37 °C. All values are presented as the means ± SDs of triplicate samples ( n = 3). All the data were analyzed via two-way ANOVA, followed by Dunnett’s multiple comparisons test. The data represent a significant difference compared to the positive control, with the p values **** p < 0.0001

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: Antimicrobial activity of AWME3 against the A. baumannii ATCC 19606 strain. The IZ values were measured after 12 h and 24 h at 37 °C. All values are presented as the means ± SDs of triplicate samples ( n = 3). All the data were analyzed via two-way ANOVA, followed by Dunnett’s multiple comparisons test. The data represent a significant difference compared to the positive control, with the p values **** p < 0.0001

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Activity Assay, Positive Control

Effect of AWME3 and P/S on A. baumannii ATCC 19606 growth curves. Bacterial cells were treated with different concentrations of either AWME3 ( a ) or P/S ( b ). Absorbance at a wavelength of 600 nm (OD 600 ) was measured at 2 h intervals for a total duration of 24 h. The experiments were conducted in triplicate, and each data point represents the average of three independent assays ± standard deviation of the mean (SD), and * p < 0.05 was considered significant

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: Effect of AWME3 and P/S on A. baumannii ATCC 19606 growth curves. Bacterial cells were treated with different concentrations of either AWME3 ( a ) or P/S ( b ). Absorbance at a wavelength of 600 nm (OD 600 ) was measured at 2 h intervals for a total duration of 24 h. The experiments were conducted in triplicate, and each data point represents the average of three independent assays ± standard deviation of the mean (SD), and * p < 0.05 was considered significant

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Standard Deviation

Effect of AWME3 extract on A. baumannii ATCC 19606 cell viability. Cell viability (%) was assessed using Alamar blue assay. The error bars represent the standard deviation of the percent viability obtained from three independent experiments, and one-way ordinary ANOVA with Dunnett’s multiple comparisons test (**** p < 0.0001) was used for significance. ( A ) Treatment with P/S; ( B ) treatment with AWME3. The MIC50 values were calculated via nonlinear regression by GraphPad Prism 7. The MIC50 values are the average of three independent experiments ± standard deviation error of the mean (SEM)

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: Effect of AWME3 extract on A. baumannii ATCC 19606 cell viability. Cell viability (%) was assessed using Alamar blue assay. The error bars represent the standard deviation of the percent viability obtained from three independent experiments, and one-way ordinary ANOVA with Dunnett’s multiple comparisons test (**** p < 0.0001) was used for significance. ( A ) Treatment with P/S; ( B ) treatment with AWME3. The MIC50 values were calculated via nonlinear regression by GraphPad Prism 7. The MIC50 values are the average of three independent experiments ± standard deviation error of the mean (SEM)

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Alamar Blue Assay, Standard Deviation

Activity of AWME3 at MIC and 2 MIC on the reduction in salt tolerance of A. baumanni ATCC 19606. All values are presented as the means ± SDs of triplicate samples ( n = 3). The data were analyzed by two-way ANOVA, followed by Dunnett’s multiple comparison test. The data displayed a significant difference compared to those of the control groups without NaCl, and p < 0.05 was considered significant

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: Activity of AWME3 at MIC and 2 MIC on the reduction in salt tolerance of A. baumanni ATCC 19606. All values are presented as the means ± SDs of triplicate samples ( n = 3). The data were analyzed by two-way ANOVA, followed by Dunnett’s multiple comparison test. The data displayed a significant difference compared to those of the control groups without NaCl, and p < 0.05 was considered significant

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Activity Assay, Comparison, Control

SEM analysis of AWME3 on A. baumannii ATCC 19606. Bacterial cells were exposed to ( a ) vehicle (PBS) - Control sample, or AWME3 at concentrations: ( b ) 0.5 MIC (0.19 mg/mL) for 12 h; ( c , d , e , f ) MIC (0.38 mg/mL) for 20 min; ( g , h , i ) 2 MIC (0.75 mg/mL) for 10 min

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: SEM analysis of AWME3 on A. baumannii ATCC 19606. Bacterial cells were exposed to ( a ) vehicle (PBS) - Control sample, or AWME3 at concentrations: ( b ) 0.5 MIC (0.19 mg/mL) for 12 h; ( c , d , e , f ) MIC (0.38 mg/mL) for 20 min; ( g , h , i ) 2 MIC (0.75 mg/mL) for 10 min

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Control

TEM analysis of AWME3 on A. baumannii ATCC 19606. Bacterial cells were exposed to ( a ) vehicle (PBS) - Control sample, or AWME3 at concentrations: ( b ) 0.5 MIC of (0.19 mg/mL) for 12 h; ( c , d , e ) MIC (0.38 mg/mL) for 1 h; ( f ) 2 MIC (0.75 mg/mL) for 30 min.; ( g , h ) 4 MIC (1.5 mg/mL) for 10 min

Journal: BMC Microbiology

Article Title: Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii

doi: 10.1186/s12866-026-04708-2

Figure Lengend Snippet: TEM analysis of AWME3 on A. baumannii ATCC 19606. Bacterial cells were exposed to ( a ) vehicle (PBS) - Control sample, or AWME3 at concentrations: ( b ) 0.5 MIC of (0.19 mg/mL) for 12 h; ( c , d , e ) MIC (0.38 mg/mL) for 1 h; ( f ) 2 MIC (0.75 mg/mL) for 30 min.; ( g , h ) 4 MIC (1.5 mg/mL) for 10 min

Article Snippet: These findings indicate that AWME3 displays high bactericidal activity compared to P/S against A. baumannii ATCC 19606.

Techniques: Control